The American Civil Liberties Union filed the federal lawsuit against the Lake County Schools on Thursday. Lake County is northwest of Orlando.
Earlier this year, the ACLU sued the district after officials refused to approve a gay-straight alliance club at Carver Middle School. A short time later, the school board agreed to a settlement allowing the club to form.
But the board passed new rules for school clubs and required all existing clubs to reapply in order to meet.
The application for a gay-straight club at Carver Middle School was rejected.
A district spokesman says they haven’t seen the lawsuit.